Culture, Differentiation, and Treatment of 3T3-L1 Preadipocytes

NB Nuria Barbarroja
SR Sergio Rodriguez-Cuenca
HN Heli Nygren
AC Antonio Camargo
AP Ana Pirraco
JR Joana Relat
IC Irene Cuadrado
VP Vanessa Pellegrinelli
GM Gema Medina-Gomez
CL Chary Lopez-Pedrera
FT Francisco J. Tinahones
JS J. David Symons
SS Scott A. Summers
MO Matej Oresic
AV Antonio Vidal-Puig
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Cells were differentiated into adipocytes (day 9) accordingly to the protocol described by Roberts et al. (17) with or without rosiglitazone 0.1 µmol/L. Lipid accumulation was assessed by Oil Red O solution (18).

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