144 healthy, adult male albino rats (Charles-Foster strain) weighing 200–250 g (6–8 weeks of age) were used in this study. Animals were housed individually in polypropylene animal cages with food pellet and water ad libitum. The animal room was maintained at the temperature of 25 ± 1°C with a 12 h light dark cycle (light 7 a.m. to 7 p.m.). According to the regulations set by institutional animal ethical committee, all adequate measures were taken to minimize the pain and discomfort in the rats.
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