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Mammary tumors were induced by a single oral gavage of 7,12-dimethylbenz[a]anthracene (DMBA) in sesame seed oil (65 mg/kg) administered to female SS, SS-3BN, line A, and line B rats at 49–55 days-of-age. Rats were palpated every week to record tumor incidence and latency. At 15 weeks, rats were euthanized and tumors were collected, weighed, and snap-frozen for further analysis.
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