RNAi

Non-targeting, HDAC1, HDAC2, HDAC3, HDAC6, XRCC4, XRCC5, XRCC6, PARP3, PNKP, BRCA2, MGMT, ELK1 and ELK3 siRNA pools were purchased from GE Healthcare/Dharmacon (D-001810–10, L-003493–00, L-003495–02, L-003496–00, L-003499–00, L-004494–00, L-010491–00, L-005084–00, L-009297–00, L-006783–00, L-003462–00, L-008856–01, L-003885–00, L-010320–00, respectively). SiRNAs were transfected into cells using RNAiMax lipofectamine from Invitrogen. Cells were transfected approximately 24 hours before starting proliferation experiments. Control, HDAC1, HDAC2, HDAC3, and HDAC6 shRNAs were purchased from Sigma-Aldrich (SHC016, SHCLNG-{"type":"entrez-nucleotide","attrs":{"text":"NM_004964","term_id":"1519499555","term_text":"NM_004964"}}NM_004964, SHCLNG-{"type":"entrez-nucleotide","attrs":{"text":"NM_008229","term_id":"87162463","term_text":"NM_008229"}}NM_008229, SHCLNG-{"type":"entrez-nucleotide","attrs":{"text":"NM_010411","term_id":"89257351","term_text":"NM_010411"}}NM_010411 and SHCLNG-{"type":"entrez-nucleotide","attrs":{"text":"NM_006044","term_id":"1653962518","term_text":"NM_006044"}}NM_006044, respectively).