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Clonogenic assay
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Panc02 cells (N = 2000) were plated into each well of 6-well plates. Old media were removed and fresh media were applied after 2 days. After 4 days, the cells were stained and fixed with methylene blue (10 mg/ml) in 50% methanol for 10 min and rinsed with distilled water. The plates were scanned and colony intensity % and area % were quantified using ImageJ software.
Copyright and License information: Springer-Verlag GmbH Germany, part of Springer Nature ©2019
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