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Fluorescent Protein Expression.
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EGFP, EBFP, and tdTomato were synthesized and characterized according to reported protocols.73 In short, Escherichia coli strain BL21 was transformed with plasmids containing corresponding recombinant proteins. After growth in 2X YT media and induction, cells were lysed and purified by Co2+ nitrilotriacetate columns. Fluorescent proteins were further characterized by 12% SDS-PAGE gel, scanning absorbance and emission spectrum. The results are consistent with previously reported work.37
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