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Twelve of the 26 tumor samples with discrepant calls between algorithm and pathologist, specifically those with algorithm-predicted MMR-D, a pathologist call of indeterminate, and sufficient leftover DNA, underwent orthogonal, tumor-only PCR testing. MSI PCR was performed using a five-marker microsatellite panel and capillary electrophoresis as previously described.11 Results were interpreted by the pathologist according to revised Bethesda guidelines.17
Copyright and License information: ©2020 by American Society of Clinical Oncology
Creative Commons Attribution Non-Commercial No Derivatives 4.0 License: https://creativecommons.org/licenses/by-nc-nd/4.0/
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