Studies of Tc13Tul effects in vivo

Six-week-old male naïve BALB/c mice were inoculated intraperitoneally (i.p.) with a daily dose of 1 μg per mouse per dose in PBS of Tc13Tul or MBP for 3 days. A control group was injected with the vehicle PBS. Sera were collected prior to inoculation and on days 4, 8 and 11 after the first inoculation from the facial vein. Eleven days after the first inoculation, mice were sacrificed and splenocytes were collected to be cultured in vitro.