Cell proliferation assay

About 2500 – 3000 cells were seeded in 96-well plates in growth media and incubated overnight at 37°C before adding serially diluted crizotinib, ceritinib, lorlatinib, cabozantinib, repotrectinib and appropriate controls. After treating the drugs, cells were incubated at 37°C for 72 hours before performing Cell Titer Glo (Promega, WI, USA) following the manufacturer’s protocol. Dose-response curves and IC50 values were calculated using GraphPad Prism.

Colony forming assays were performed by seeding the cells in 6-well plates in growth media with appropriate drug-containing media replaced every three days. Plates were fixed in 4% PFA and stained in crystal violet for 1 hour after 14 days of drug treatment.