Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Yeast strains with GFP-tagged proteins and microscopy

PT Peter Tsvetkov
TE Timothy J. Eisen
SH Sven U. Heinrich
ZB Zarina Brune
EH Erinc Hallacii
GN Greg A. Newby
CK Can Kayatekin
DP David Pincus
SL Susan Lindquist
request Request a Protocol
ask Ask a question
Favorite

yeast strains with Om45,Tom70, Vph1, Pma1, Sec63, Pab1, Dcp2 and Hsp104 GFP tagged were received from Dirk Landgraf (Landgraf et al., 2016). For the co-localization experiments yeast cells harboring the mCherry tagged VMA1 were mated to the GFP reporter cells. The resulting diploid cells were analyzed for colocalization. All images were acquired with a Nikon Plan Apo 100x oil objective (NA1.4) using a Nikon Eclipse Ti-E inverted microscope and a CCD camera (Andor technology). Images were analyzed using ImageJ software.

Copyright and License information:  ©2020

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A