Stimulation Parameters

Single pulse electrical stimulation was performed using bipolar stimulation between adjacent SEEG contacts with a symmetrical biphasic charged-balanced waveform, without an interphase delay, with standard parameters¹² using a Natus Nicolet Cortical Stimulator (Natus Neurology Incorporated, WI). Parameters for stimulation were 300 μs/phase pulse width delivered at 1 Hz for 10–20 seconds per train and stimulus intensity titrated in 1 mA increments from 1.0 mA up to a maximum current of 6.0 mA for AdTech electrodes and 5.0 mA for Dixi electrodes though we only analyzed the two runs with maximal charge from each stimulation site). The maximum charge density (6 mA * 0.3 mS / 0.0618 cm² = 29 μC/cm² for AdTech; 5 mA * 0.3 mS / 0.05 cm² = 30 μC/cm² for Dixi) was chosen to match the recommended safety limit extrapolated from clinical deep brain stimulation (30 μC/cm²)¹⁰. Stimulation was applied to a variety of contact pairs of clinical interest, including both the medial and lateral temporal lobe.