Gene expression across temporal stages of prostate development.

Intensity of signal for each of the 16,047 mCRPC-enriched H3K27ac loci was ranked by adjusted P-value and fold change. The top 100 sites that had overlap with a TSS was selected. All human genes with a homologous mouse gene were converted and used in the analysis.

Raw FHCRC Mouse Prostate MPEDB cDNA Array data was downloaded from GEO ({"type":"entrez-geo","attrs":{"text":"GSE19225","term_id":"19225"}}GSE19225), expression values for the genes described above were evaluated. Levels were measured relative to expression at embryonic day 14 as described³⁴.

Box-and-whisker plots depicting the median, 25th–75th percentile interval and extremes in expression across the temporal stages of prostate development were calculated. P-value was determined by Wilcoxon signed-rank test⁹², comparing embryonic and post-natal samples.