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Formaldehyde stability (Fig. 1e)

MP Matthias Pietzke
GB Guillermo Burgos-Barragan
NW Niek Wit
JT Jacqueline Tait-Mulder
DS David Sumpton
GM Gillian M. Mackay
KP Ketan J. Patel
AV Alexei Vazquez
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In total, 40 µM of 13C-formaldehyde was added to complete IMDM, including 10% FBS, and 1 ml of this medium was added to blank wells of a 12-well plate or to wells in which 100.000 HAP1-wt cells were seeded the day before the experiment. Plates were cultured in an incubator at 37 °C in a humidified 5% CO2 atmosphere. At the indicated time points, the medium was harvested, cells spun down and the supernatant stored at −80 °C, before 100 µM of this were used for the formaldehyde quantification. The experiment was performed three times, with three replicates per experiment.

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