Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Quantitative reverse transcriptase PCR.

EM Emily M. Mace
VB Venetia Bigley
JG Justin T. Gunesch
IC Ivan K. Chinn
LA Laura S. Angelo
MC Matthew A. Care
SM Sheetal Maisuria
MK Michael D. Keller
ST Sumihito Togi
LW Levi B. Watkin
DL David F. LaRosa
SJ Shalini N. Jhangiani
DM Donna M. Muzny
AS Asbjørg Stray-Pedersen
ZA Zeynep Coban Akdemir
JS Jansen B. Smith
MH Mayra Hernández-Sanabria
DL Duy T. Le
GH Graham D. Hogg
TC Tram N. Cao
AF Aharon G. Freud
ES Eva P. Szymanski
SS Sinisa Savic
MC Matthew Collin
AC Andrew J. Cant
RG Richard A. Gibbs
SH Steven M. Holland
MC Michael A. Caligiuri
KO Keiko Ozato
SP Silke Paust
GD Gina M. Doody
JL James R. Lupski
JO Jordan S. Orange
request Request a Protocol
ask Ask a question
Favorite

RNA was extracted from enriched NK cells or secondary lymphoid cells (SLC) using DirectZol (Genesee Scientific) or RNeasy (Qiagen), and cDNA was made using VILO (Invitrogen). IRF8, PRDM1, NFIL3, and IRF4 were amplified on a Roche Light Cycler by Taqman gene expression array. GAPDH was used to normalize expression for each sample. Each condition was performed in quadruplicate. Analysis was performed with Roche Light Cycler software and exported to Excel before graphing with Prism 6.0 (GraphPad software).

Copyright and License information:  ©2017, American Society for Clinical Investigation

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A