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Bacterial strains and growth conditions
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Bacterial strains and plasmids used in this study are identified in Table 1. All strains were maintained at −80°C in Luria-Bertani (LB; 1% tryptone, 0.5% yeast extract and 1% NaCl, w/v) broth containing 20% (v/v) glycerol. Single colonies were grown in LB broth at 37°C. Ampicillin was used at a concentration of 100 μg/ml to select for and maintain plasmids.
Bacterial strains and plasmids used in this study
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