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Variable domain amino acid sequence alignment
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The variable domains, including both VH and VL, from various APA clones found in the literature were compared by multiple amino acid sequence alignment using Clustal Omega. Residues were numbered according to Kabat numbering, and CDRs were identified using the abYsis online resource. APA clones 6-3, 3-3, E11, PEG.2 6A9, and 157G29D1 are specific to the PEG repeating monomer backbone as a binding epitope, while APA clone 15-2 is specific to the terminal methoxy-PEG (mPEG) end group as a binding epitope.
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