Animals and compound application

Nine male RjOrl:Swiss (CD-1) mice (38–41 g body weight, purchased from Janvier Labs, France) were used for the pilot in vivo study. The animals were housed in a temperature-controlled room (20–24 °C) and maintained in a 12 h light/12 h dark cycle. Food and water were available ad libitum. The in-life phase was performed by the contract research organization Pharmacelsus (Saarbrücken, Germany). All experimental procedures were approved by and conducted in accordance with the regulations of the local Animal Welfare authorities (Landesamt für Gesundheit und Verbraucherschutz, Abteilung Lebensmittel- und Veterinärwesen, Saarbrücken). Six animals received a single oral dose of 10 mg/kg body weight of 41, and three animals received the vehicle. Water containing 1% hydroxypropyl methylcellulose (HPMC)/Tween 80 (99:1) served as vehicle. All animals behaved normal throughout the study and showed no adverse effects.