Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Measurement of cellular fluorescence intensity

AN Atsushi Nakayama
AO Akira Otani
TI Tsubasa Inokuma
DT Daisuke Tsuji
HM Haruka Mukaiyama
AN Akira Nakayama
KI Kohji Itoh
AO Akira Otaka
KT Keiji Tanino
KN Kosuke Namba
ask Ask a question
Favorite

Digital imaging analysis was also performed by a standard imaging software attached to the confocal microscopic system in a fluorescence mode. The cells containing significant fluorescent inclusions were designated as positive cells. Three different areas showing 20–30 cells each were viewed. The relative amount of the accumulated natural substrates in microglial cell population was expressed as immunofluorescence intensity index in each image (mean value). Relative fluorescence intensity was graded, ranging from 0 to 255 in this system. The fluorescence intensity was linearly correlated with the number of pixels showing a positive signal. Relative intensities for other cell strains were calculated within the range of this gradation.

Copyright and License information: The Author(s) ©2020
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A