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For inhibitor treatment, 200 μg/mL of soybean-derived serine protease inhibitor (VWR AMRESCO, K213-1G; Avantar, Radnor, PA) or 30 μmol/L chymostatin (MP Biomedicals, Irvine, CA) was applied to LUVAs for 1 hour. Before adding to endothelial cells, the LUVA cells were washed with Dulbecco's phosphate-buffered saline and added to the in vitro angiogenesis experiments.
Copyright and License information: ©2020 American Society for Investigative Pathology. Published by Elsevier Inc.
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