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MTGase-producing bacterium Streptomyces sp. TYQ1024 (GeneBank Accession No. MN606211) was maintained in our laboratory. Escherichia coli DH5α (Vazyme, China) was used for vector construction. Bacillus subtilis SCK6 (BGSC 1A976) (Erm R, his, nprR2, nprE18, ΔaprA3, ΔeglS102, ΔbglT/bglSRV, lacA::PxylA-comK) was used as the expression host [24]. pMA0911 with Tat (Ywb N, Lip A, Amy X, Wap A) and Sec (Ync M, Npr E, Vpr, Yvg O) signal peptides was used as the expression vector.
Copyright and License information: ©2020 The Korean Society for Microbiology and Biotechnology
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