Analysis of Purified AGP by SDS-PAGE

SDS-PAGE polyacrylamide gels were used to confirm the purity of the purified AGP, and the fractionated AGP was visualized by silver staining. The samples were diluted in phosphate-buffered saline (PBS) and subjected to 12.5% polyacrylamide gel electrophoresis with sodium dodecyl sulfate under reducing conditions. The molecular weights and purities of the various AGP fractions were assessed using commercial AGP from human plasma as standard (Sigma-Aldrich, USA, Cat. No. G9885).