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A 0.25 g sediment sample was collected, and then genomic DNA from each sediment sample was extracted in triplicate using a DNA Isolation Kit (E.Z.N.A., Omega, USA) in accordance with the manufacturer’s instructions. Subsequently, the concentration and purity of genomic DNA were detected with an ultraviolet spectrophotometer, and verified by 1% agarose gel electrophoresis. The measured DNA sample was stored at -20°C for 16S rRNA gene PCR amplification and sequence analyses [40].
Copyright and License information: ©2020 The Korean Society for Microbiology and Biotechnology
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
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