Infectious progeny experiments

C. trachomatis infections with inhibitor treatment were performed as described above, but in 24-well plates. At 40 h post infection (hpi), infected cells were isolated by scraping and collecting in the original culture media and were stored at −80 °C until further analysis by titer assay. Samples were thawed, vortexed and serially diluted (1 : 10). Diluted samples were used to infect new HeLa cells seeded in 96-well plates. At 40 hpi, the previously described immunofluorescence staining and imaging protocol was employed to count recoverable IFUs. Data are shown as the average infectious progeny for a treatment condition relative to that of the ethanol vehicle control ± SEM.