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T cells were enriched from the lymph nodes (LNs) of WT and Runx3KO mice by negative selection using anti-mouse IgG BioMag beads (Qiagen). The purity of the isolated cells was confirmed by flow cytometry. The LN T cells were resuspended in IMDM media at 1–2 × 106 cells/mL and stimulated with 5 ng/mL TGFβ (R&D Systems) for 4 days at 37 °C. On day 4, stimulated cells were analyzed by flow cytometry.
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