2.2. Animals

Adult male Wistar Han rats (Envigo, Indianapolis, IN, USA) were housed in groups of twos or threes with ad libitum access to water and were food restricted to maintain a maximum weight between 350–400 g. Rats were kept under a 12-hr light/dark cycle with experiments taking place in the light phase and were acclimatized to all experimental procedures to reduce stress. All animal procedures conformed to the ARRIVE guidelines (Percie du Sert et al., 2020), the recommendations of the National Research Council’s Guide for the Care and Use of Laboratory Animals, and the Canadian Council on Animal Care, and were approved by the Animal Care Committee at the University of Toronto.