2.10. In Vivo Bioluminescence Imaging and Quantification of Tumor Burden.

Intracranial tumor growth was monitored via bioluminescence imaging. Briefly, animals were anesthetized in an induction chamber with 2.5% isoflurane and intraperitoneally injected with Pierce D-Luciferin (150 mg/kg, dissolved in PBS) (ThermoFisher Cat# 88293). After 10 min, animals were moved to a Xenogen IVIS system (Caliper Life Sciences, Hopkinton, MA) maintained at 2.5% isoflurane and imaged for tumor bioluminescence. Photons emitted from live mice were acquired as photons/s/cm2/steradian (p/s/cm2/sr). The average radiance (p/s/cm2/sr) within identical regions of interest drawn around the brain was used to quantify tumor burden using Living Image software (PerkinElmer, MA).