Ferric chloride–induced arterial thrombosis

Mice were given intraperitoneal anesthesia (ie, medetomidine 0.5 mg/kg, midazolam 5 mg/kg, fentanyl 0.05 mg/kg), and injected with 100 µg i[VREY]₄ or an equimolar amount of VREY control (20 µg) in phosphate-buffered saline (PBS) or PBS alone (200 µL each) 1 hour before the procedure. Carotid artery thrombosis was induced by 10% ferric chloride (FeCl₃) and blood flow monitored by Doppler sonography, as detailed in the supplement.