2.9. Perfusion through Bioprinted Vessels.

After 3D bioprinting of hollow vessels, the constructs were removed carefully with stainless steel tweezers and washed with PBS. A platform was prepared on a Petri dish, where the vessel was placed in the center. Plastic tubing with 1 mm of the inner tube diameter was connected to the inlet and outlet of the vessel supported over a small slice of PDMS and pasted on the edges of the cylinder with a Loctite Ultra Gel Control Super Glue. A 1 mL syringe containing red food dye in DPBS was connected to the inlet plastic tube and flowed slowly. DPBS was passed through the vessel without any leakage for 1 min of a continuous, manual hand-controlled flow.