Lung cellular superoxide measurements

Lung tissue was also obtained from the mice injected with CPH as described above to detect lung cellular superoxide. Following collection of blood and euthanasia, the chest was opened, and lungs were flushed with 10 ml cold PBS via the right ventricle to remove blood. 10 mg of lung tissue was cut, weighed and placed in a tissue cell, an accessory used to detect EPR signal from tissues (Bruker BioSpin). EPR measurements were performed at RT. The tissue cell accessory was cleaned with ethanol between samples.