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Human colon cancer epithelial RKO cells (ATCC CRL-2577) were grown in McCoys 5A with L-glutamine (Corning) supplemented with 10% (v/v) fetal bovine serum (Sigma). Antibiotics (100 U/mL penicillin, 100 μg/mL streptomycin; Gibco) were included in the growth medium during maintenance of cell lines and removed 24 h prior to bacterial co-culture. Cell cultures were incubated at 37°C in 5% CO2.
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This is an open access article under the CC BY-NC-Nd license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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