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First, rats were anesthetized with intraperitoneally-injected 0.3% sodium pentobarbital (30 mg/kg) and decapitated. The collection of hippocampal tissues was performed on ice, homogenized with a homogenizer, and centrifuged for 10 min at 10,000 rpm at 4 °C. Next, 1% of lysates were subjected to ELISA following the manufacturer’s instructions. The expression levels of Aβ1-42 were evaluated using a microplate reader at 450 nm.
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