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NucBlue Live Ready Probes Reagent (ThermoFisher Scientific, Waltham, MA, USA) is a room temperature-stable live cell stain that contains Hoechst 33342, a cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. In order to minimize cell toxicity, the 1X protocol (2 drops/mL) was diluted to 0.1X (2 drops/10 mL). At DIV7, the cells were fed with 50% feeding media + 0.1X NucBlue prior to imaging.
Copyright and License information: ©2017 by S. Karger AG, Basel
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