Arabidopsis Col and dorn1‐1 (both constitutively expressing cytosolic (apo)aequorin) were grown on full or zero Pi medium for 11 days. Plates were scanned using an Epson scanner with 300 dpi resolution. ImageJ software with the NeuronJ plugin was used to quantify primary root length (the 8‐ to 11‐day interval).
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