Cell Viability

MTT assay was used to determine the viability of cultured cells following manufacturer instructions. 1 × 10⁴ cells were plated in 96 well cell culture plate in 200 µl of medium and incubated with varying concentrations (0.125–1 µg/ml) of drug docetaxel alone and PLGA-Dtx nanoconjugate containing the same amount of drug as equivalent to the free drug for 24 h. The range of the docetaxel concentration has been selected based on previous literature [16, 17]. After incubation for desired time 10 µl of MTT solution was added to each well and waited for the next 3 h. The cell viability was assessed at 550 and 660 nm utilizing a microplate reader.