Caspase 3/7 activity assay

ZC Zhenyu Chen
EC Eric Chai
YM Yongchao Mou
RR Ricardo H Roda
CB Craig Blackstone
XL Xue-Jun Li
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Caspase 3/7 activities were measured using the Caspase-Glo 3/7 Assay’s (Promega) protocol. About 5000 cells were dissociated into single cells and mixed with 50 µl caspase 3/7 reagent in one well of a 96-well plate. After 1 h at room temperature, luminescence was measured using a BioTek Synergy2 Multi-Mode Microplate Reader.

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