Total cholesterol, triglycerides and LDL were evaluated at sacrifice by Ospedale San Raffaele Mouse Clinic (Milan - Italy). Circulating cytokines, chemokines and hormones were measured on stored samples by using Bio-Plex assays (Pro Mouse Diabetes Assay, Mouse Group I: 23-plex panel, Mouse Group II: 9-plex panel and Mouse Adiponectin assay) following manufacturer’s instruction using Bio-Plex 200 Suspension Array System (Biorad). Circulating apoE protein was measured by ELISA assays (mouse apoE Elisa Kit (USCN Life Science Inc.)).
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