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The potential relationships between variables were determined using Spearman correlation coefficient. The t-test (normally distributed data) and Wilcoxon rank-sum test (non-normally distributed data) were used for two group comparison. Additionally, Kruskal–Wallis and one-way analysis of variance (ANOVA) were employed for within-group and multiple-group comparisons. A two-tailed P-value of 0.05 was used as the significance criterion for all data analyses.
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