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YX Yue Xu
HZ Haifeng Zhang
YC Yuxin Chen
JP Jordan S. Pober
MZ Min Zhou
JZ Jenny Huanjiao Zhou
WM Wang Min
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Mice were housed in the animal care facility of Yale University under standard pathogen-free conditions (temperature, 20–24 °C; relative humidity, 45–65) with a 12 h light/dark schedule and provided with food (D10001, AIN-76A Rodent Diet; Research Diets, Inc.) and water ad libitum. Both male and female adult mice were used in all experiments without significant differences between male and female within the same group and we presented data primarily from the male mice. We used a previously generated conditional gene knockout mouse model lacking Senp1 specifically in VSMCs (Senp1SMCKO)24. We used EC-specific Senp1 deletion line (Senp1ecKO) as control22–25. The carotid arteries of C57BL/6, Senp1lox/lox (WT) and Senp1SMCKO mice (both having C57BL/6 background for more than six generations) were genotyped by western blot, quantitative RT-PCR, and immunofluorescence staining.

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