Cell Proliferation Assay (MTT)

In this study, the extracts from Aspergillus flavus isolate, and Aspergillus niger were investigated for their potential anticancer effects on the mentioned cell lines. The cells were exposed to varying concentrations of each extract, ranging from 0 to 1000 µg/mL. Doxorubicin, a chemotherapy drug, and mitoxantrone, an antineoplastic agent, were used as positive controls for assessing cell toxicity, while cells treated with Dimethylsulfoxide (DMSO) only were used as a negative control. After 48 hours, the cells were treated with 5 mg/mL MTT solution. Following a 3-hours incubation at 37 °C, the media was removed, and 100 µL of DMSO solvent was added to each well. After shaking the plate on an orbital shaker for 45 minutes, the absorbance was measured at OD 590 nm. The half-maximal inhibitory concentration (IC50) values for each substance were determined using the dose‒response curve in GraphPad Prism 8. Each experiment was repeated three times for accuracy.