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Tissue slides were cropped into 5 μm slices and subjected to dewaxing, H&E and IHC analysis were carried out as previously described. Briefly, slices were incubated with antibodies against CD4, CD8, TNF-α, and IFN-γ (Bioss, Beijing, China). Subsequently, they were subjected to DAB staining and scanned by 3D Histech. Representative pictures were shown from each treated group.
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