Reagents

Anti-Flag (Cat# M20008), anti-GFP (Cat# M20004), anti-GLP1 (Cat# T56696), and anti-HA (Cat# M20003), anti-INSR (Cat# T55803), anti-IRS1 (Cat# T55830), anti-pIRS1 (Cat# TA3272), anti-IRS2 (Cat# PA5455), anti-pIRS2 (Cat# TA8383), anti-AKT (Cat# T55561), anti-pAKT (Cat# T40067), anti-PERK (Cat# TP52759), anti-pPERK (Cat# TA4499), anti-IRE1 (Cat# TA7651), anti-pIRE1 (Cat# T55605), anti-ATF4 (Cat# T55873), anti-XBP1 (Cat# T56725) antibodies were purchased from Abmart. Anti-cleaved caspase3 (Cat# 9664) antibody was purchased from Cell Signaling Technology. Anti-Nogo (Cat# IMG-5346A) antibody was purchased from Novus. Anti-GAPDH (Cat# HRP-60004), anti-pINSR (Cat# 31133-1-AP), anti-GCG (Cat# 15954-1-AP), anti-GRP94 (Cat# 14700-1-AP), anti-HSP90 (Cat# 13171-1-AP), anti-Rabbit IgG (Cat# 30000-0-AP), fluorescein (FITC)–conjugated Affinipure Goat anti-Mouse IgG(H + L) (Cat# SA00003-1), fluorescein (FITC)–conjugated Affinipure Goat anti-Rabbit IgG(H + L) (Cat# SA00003-2), Rhodamine (TRITC)–conjugated Goat anti-Mouse IgG(H + L), (Cat# SA00007-1), and Rhodamine (TRITC)–conjugated Goat anti-Rabbit IgG(H + L) (Cat# SA00007-2) antibodies were purchased from Proteintech. Anti-insulin (Cat# sc-377071) and anti-PCSK1 (Cat# sc-100578) antibodies were purchased from Santa Cruz Biotechnology.

DMEM (Cat# 01-052-1 A) and Fetal bovine serum (Cat# 04-010-1 A) were purchased from Biological Industries. 4% formaldehyde (Cat# BL539A), Penicillin-Streptomycin Solution (Cat# BL505A), Total RNA Isolation Reagent(Cat# BS258A), Cell lysis buffer (Cat# BL509A) and Commassie Brilliant Blue Staining Solution (Cat# BL605A) were purchased from Biosharp. EntransterTM-in vivo (Cat# 18668-11-1) was purchased from Engreen Biosystem. DAPI (Cat# HY-D0814) was purchased from MCE. Streptozotocin (STZ, Cat# 572201) and Oil red O (Cat# O9755) were purchased from Sigma-Aldrich. Insulin (Cat# I8830) and D-glucose (Cat# G8150) were purchased from Solarbio. Hieff TransTM Liposomal Transfection Reagent (Cat# 40802ES03) and GoldBand Plus 3-color Regular Range Protein Marker (Cat# 20350ES) were purchased from Yeasen Biotechnology. rProtein A/G MagPolyBeads (Cat# A10002) was purchased from Abmart. Mouse GLP1 Elisa kit (Cat# RX203401M), Mouse glucagon Elisa kit (Cat# RXJ202477M), and Mouse insulin Elisa kit (Cat# RX202485M) were purchased from Ruixin Biological Technology. Triglyceride labassay (Cat# 290-63701) was purchased from Wako Chemical. High Fat Diet 60% (Cat# MD12015A) was purchased from Medicience. Urea (Cat# U5128), dithiothreitol (DTT) (Cat# D9779) and iodoacetamide (IAA) (Cat# I1149) were purchased from Sigma. Sequencing grade modified trypsin (Cat# V5111) was purchased from Promega. Sep-Pak C18 cartridges (Cat# WAT023590) were obtained from Waters.