Cell cycle analysis by flow cytometry

HO Hiroshi Onji
ST Sota Tate
TS Tomohisa Sakaue
KF Kohei Fujiwara
SN Shiho Nakano
MK Miho Kawaida
NO Nobuyuki Onishi
TM Takashi Matsumoto
WY Wataru Yamagami
TS Takashi Sugiyama
SH Shigeki Higashiyama
YP Yves Pommier
YK Yusuke Kobayashi
JM Junko Murai
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Cells were incubated with 10 μM EdU for 1 h using a Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (cat. no. C10419; Invitrogen) according to the manufacturer’s instructions. Propidium iodide (cat. no. P4170; Sigma-Aldrich, St. Louis, MO, USA) was used to measure DNA content. Data were collected with a Gallios Flow Cytometer and analyzed with FlowJo software.

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