Cells were incubated with 10 μM EdU for 1 h using a Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (cat. no. C10419; Invitrogen) according to the manufacturer’s instructions. Propidium iodide (cat. no. P4170; Sigma-Aldrich, St. Louis, MO, USA) was used to measure DNA content. Data were collected with a Gallios Flow Cytometer and analyzed with FlowJo software.
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