2.5.1. Effects of Different Carbon Sources on Growth and mcl-PHA Production

The impact of various carbon sources on both the growth and polyhydroxyalkanoate (PHA) production of the bacterial culture was investigated. A 100 mL volume of sterilized nutrient broth media (NB) was enriched with different carbon sources, waste cooking oils (olive, palm, and sunflower oils), olive oil, potato starch, glucose, and glycerol at a concentration of 20 g/L. The composition of the nutrient broth was as follows (g/L): peptone, 5; NaCl, 5; yeast extract, 1.5; beef extract 1.5. To prepare 100 mL of nutrient broth, 1.3 g of nutrient broth powder (purchased from Hi-Media, Thane, India) was suspended in 100 mL of distilled water. The pH of each medium was adjusted to 7.0 using sodium phosphate buffer saline (PBS). The pure bacterial isolate was inoculated in each well, after which the mixture was subsequently incubated at 37 °C in an orbital incubator (New Brunswick Innova 44, Eppendorf, Hamburg, Germany) at 150 rpm for 24 h. Measurements of both the biomass and PHA content were taken from the culture broth after 48 h of incubation.