4.2. Animals

Male mice strain CD-1 were obtained from the Institute of Health Sciences at the Autonomous University of Hidalgo (Hidalgo, Mexico) and maintained according to the norms of the Institutional Ethics Animal Care and Use Committee (CIECUAL) under standard conditions with a 12 h/12 h light/dark cycle, constant temperature (22 ± 2 °C), and humidity (50 ± 10%). Food (Lab Diet^® (St Louis, MO, USA) 5013) and water (depending on the experimental group) were freely available in their home cages. The adjustment time period for the animals was one week before the treatments. The experimental procedures were approved by CIECUAL (Approval Number: CIECUAL-V-I/013/2023) of the Autonomous University of the State of Hidalgo (AUSH).