2.8.1. oADSCs Culture with Teno-Inductive CM

oADSCs at P1 and P6 were cultured in SM until reaching 70% of confluence (Figure 1(C1)). Subsequently, the cells were cultured in a teno-inductive CM 1:1 diluted with GM, according to previously established protocols [41]. The teno-inductive CM was obtained from a co-culture of oAECs with fetal tendon explants and collected according to our previous reports [18,41]. oADSCs were incubated in CM for 14 days at 38.5 °C in a 5% CO₂ atmosphere, and the medium was refreshed every 48 h up to the end of the culture. As the internal positive control (CTR int), the oAECs were used to assess the teno-inductive potential of the CM [41]. Cell morphology was monitored throughout the culture period.