Cell apoptosis analysis

CRC cells were cultured in DMEM containing different concentrations of STM2457 (0,20,40 μ M) for 48 hours. According to the instructions, the cells were collected separately and resuspended with 1×Annexin V Binding Solution, then FITC and PI solutions were added to the cell suspension. Subsequently, the suspension was placed in dark and at room temperature for 15 minutes, and flow cytometry was applied to measure cell apoptosis. The reagent used in cell apoptosis experiment is Annexin V, FITC apoptosis detection kit (DoJinDo Laboratories, Japan). The obtained results were analyzed by FlowJo software (FlowJo, LLC). All experiments were repeated at least three times.