Lactate production and glucose consumption measurement

YW Yaoxin Wu
YL Yi Luo
TL Tinghao Li
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Lactate and glucose concentrations in the culture supernatants were detected using Lactate Assay kit (Solarbio, Beijing, China) and Glucose Assay kit (Solarbio) respectively, according to the manufacturer’s instructions and absorbance values were measured at the corresponding absorbance. The results were normalized by the number of cells in each sample in the culture plates, lactate production and glucose consumption were calculated by comparison with normal medium.

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