Lactate and glucose concentrations in the culture supernatants were detected using Lactate Assay kit (Solarbio, Beijing, China) and Glucose Assay kit (Solarbio) respectively, according to the manufacturer’s instructions and absorbance values were measured at the corresponding absorbance. The results were normalized by the number of cells in each sample in the culture plates, lactate production and glucose consumption were calculated by comparison with normal medium.
Copyright and License information: The Author(s) ©2024 Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.