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Cell viability was analyzed using the MTT assay over a 7‐day period, as mentioned previously [28]. Cell lines were seeded in 48‐well plates (10 000 cells per well). Cells were then treated by a range of concentrations of Taxol® (0 to 160 nm) in their respective cell culture medium at 24 and 96 h after seeding. Cell survival rates in Taxol® conditions were normalized to control condition (without drug). An increase of EC50, the dose for which 50% of the drug's maximal effect is obtained, is associated to drug resistance.
Copyright and License information: ©2024 The Authors. published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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