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LAMP reaction and conditions
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The LAMP assay was performed in a total volume of 25 µL consisting of ultra-pure water, 1.6 µM for primers FIP/BIP, 0.2 µM for primers F3/B3, 5 mM betaine, 1.4 mM dNTPs, 6 mM MgSO4, 8 U of Bst 3.0 DNA polymerase, 2.5 µL of 10 × Bst Reaction (final 1 ×), and 1 µL of template DNA (5.2 ng/µL). The optimal conditions of the LAMP assay were determined by running at different temperatures (45, 50, 55, 60, 65, 70, 75 and 80 °C) and reaction times (15, 30, 45, 60 and 75 min).
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