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Negative stain and image acquisition of EV suspensions were performed as described previously (32). A droplet of 10 µl EV suspension was transferred to a 150 mesh Formvar coated copper grid (0150-Cu, Electron Microscopy Sciences) and incubated for 20 minutes, then drained with filter paper and stained with ammonium molybdate for 10 minutes. After draining, the grids were washed on a droplet of double distilled water for 10 seconds. Samples were imaged on a Talos F200i (Thermo Fisher Scientific).
Copyright and License information: ©2024 Dekkers, Lambooij, Pu, Fagundes, Enciso-Martinez, Kats, Giepmans, Guigas and Zaldumbide
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